The aim of this plan is evaluating NNV inactivated vaccine to induce the broodstock to produce the neutralizing antibody. We wish to reduce or block the roe of NNV vertical infection. The first year, the adult fish (average body weight 1.65 kg) was used to carry on the best vaccine immunizing dose and the condition. After immunity by two dosage's NNV inactivated vaccine ,To measure and trace the titer of neutralized antibody continuously for five months. The result showed that the titer is higher in dose of 109 TCID50 kg-1 than 108 TCID50 kg-1, and adjuvant group is better than not adjuvant group , the security of injection by intramuscular compared to abdominal cavity injection group.
The newly hybrid tilapia, bred by Freshwater Aquaculture Research Center, FRI, only need to be cultured for one year to marketable size, with high male ratio, are very suitable for the farmers.Previously, 0.1 million fry were to be produced this year, but total of 0.23 million had been already sold to 3 farmers; unfortunately, the cultured fry were all run off by the heavy rainfall from Typhoon Morakot, Fry of Nile tilapia (Oreochromis niloticus) and Blue tilapia (Oreochromis aurea), respectively, were cultured to be selected as broodstock next year.
Cultured small abalone, Haliotis diversicolor, collected from six hybrid combinations the sum of 90. RAPD and six random primers (p3、p5、p7、p8、p9、p12) were then used to analysis and amplify 81 clear and reproducible bands, then utilize Un-weighted Pair Group Method Analysis (UPGMA), when the genetic distance 0.3 can divide 90 samples into 4 groups.
Red tilapia, Oreochromis spp, collected from one hybrid (ct), inbred lines (TsR) and commercial line (cp and hcp) the sum of 60. RAPD and six random primers (p24、p31、p36、p39、p40、p48) were then used to analysis and amplify 25 clear and reproducible bands, then utilize Un-weighted Pair Group Method Analysis (UPGMA), when the genetic distance 0.22 can divide TsR line and commercial line: cp, hcp, a lot of ct.