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水產種苗研究團隊-水產種原分子生物技術輔助育種平台之建立

  • 日期:100-04-07
  • 計畫編號:99農科-1.1.2-水-A1
  • 年度:2010
  • 領域:農業生物技術研發領域
  • 主持人:曾福生

九孔
四種不同來源配對的九孔,台日(Ja1:台南品系和日本野生貝雜交)、台南品系(N)、南碕(NG:台南品系和磯碕野生貝雜交)及和南(HN:和平島野生貝和台南品系雜交),1月齡的附苗率以雜交苗,台日F1、台日F2、南碕及和南5.74~9.42%以上優於台南品系的1.2~1.54%。成長方面,雜交貝台日F2、南碕,成長最快,依序為台日F1,台南品系,和南。存活率以雜交貝,台日F1、台日F2、南碕及和南最佳97%以上,台南品系83%,結果顯示,引進種貝進行遠緣雜交能有效改善成長及活存率。
吳郭魚
以建立之紅色吳郭魚TsR品系、CP1(來自業者之吳郭魚品系)二個吳郭魚的品系配對,經由雜交組有TsR♀xCP1♂和TsR♂xCP1♀,自交組則分為TsR 品系,和CP1 品系,4種不同的吳郭魚品系組合。六月齡時體重在350g以上,正交有26.7% (16 / 60)、反交78.3% (47 / 60)、CP1自交65% (39 / 60)及TsR自交沒有超過350g。以4個家系中六月齡體重350g以上的魚進行分子標誌的篩選,有5個分子標誌A1、A2、A3、p189及p176都出現在350g以上的魚。TsR雖然沒有超過350g的魚,然而,這5個分子標誌仍出現在♂的機率高達75%以上,其中p189及p176不出現在♀,意味著成長和性別連鎖。

研究報告摘要(英)


Small abalone
Hybrids of small abalone from four different sources mated, F1: hybrid crossing female farmed from Taiwan with the wild male from Japan; F2: F1 inbred; N: inbred from farmed of Taiwan; NG crossing farmed female with wild male caught from Hualien coast in Taiwan and HN crossing wild female caught Keelung coast in Taiwan with farmed male. The survival rate of 1-month-old larvae, the hybrids were over 5.74~9.42% significantly higher N 1.2~1.54%. We compared the each strain growth rate, F2, NG, F1, N and HN in order. The survival rate of N was 83% the lowest; the others were more than 97%. These results showed that the introduction from outbreed was a significantly improvement on growing up and survival rate.

Red tilapia
The hybrids, TsR♀xCP1♂and TsR♂xCP1♀, were selected from completely diallel mating with red tilapia, Oreochromis spp., and inbred TsR and CP1. There were 4 different crosses, at 6-months-old, all body weight over 350g was chosen, TsR♀xCP1♂: 26.7%; TsR♂xCP1♀: 78.3%; inbred CP1: 65% and inbred TsR was 0%. We used all the crosses to screen molecular marker associated growth, there were 5 loci marker A1, A2, A3, p189 and p176 existing in all over 350g fish. However, the inbred TsR had not over 350g fish, the more than 75% male shared with A1, A2, A3, p189 and p176,the female was not p189 and p176. These results showed that the growth associated marker may be linkage sex determination marker.