The kit, named Total Vibrio detecting kit (TV-kit), which can be used by general consumers to detect the numbers of Vibrio spp. (including Vibrio aestuarianus、 V. alginolyticus、V. anguillarum、V. campbellii、V. fluvialis、V. furnissii、V. harveyi、 V. mimicus、V. natriegens、V. nereis、V. parahaemolyticus、V. pelagia、V. proteolyticus、 V. salmonicida、V. tubiashi、V. vulnificus及 Photobacterium damselae subsp. damselae) in different samples has been developed in this project. The distinguishing designs of this kit are friendly to use and fast easily to read the results. Moreover, the assistance by technicians or equipments is not necessary while using the kit. To handle the kit, only one drop (about 40 ul) of sample was needed to add into the tube. Positive results can be represented by color change (from colorless to red-purple) in 24 to 48 hours. The timing of colorization related to the number of Vibrio spp. in samples. For specificity analysis, 54 reference strains of fish pathogens, 10 isolates from freshwater, 20 isolates from seawater and 8 indicator bacteria to sanitation were tested with TV-kit one by one. The result confirms that only one of the 17 Vibrio spp. mentioned above can make TV-kit changing color.
As the series products of “Easy kit” developed in Fisheries Research Institute, TV-kit can not only quantitatively determine the numbers of Vibrio spp. in samples, it but also can conduct the bacterial susceptibility test to quickly screen out the correct medicine, avoid from the waste of drugs with no use and prevent the resistant strains. Using the friendly detecting tool well, even a common fish farmer can practice the sanitation management by himself.