農業部-水產試驗所全球資訊網農業部-水產試驗所全球資訊網

本研究由海洋細菌篩選得到兩株可生產還原蝦紅素之菌株，經傳統微生物生化與16S rRNA鑑定為Paracoccus sp.，編號為AS-14及AS-15，以MB培養基中分別添加0.25%蔗糖、0.5%果糖作為AS-14、AS-15菌株之較適碳源，使得還原蝦紅素產量可達0.20 mg/L及 0.71 mg/L。另一方面，以物理(UV、微波)方式突變菌株，在200 J/cm2多次照射誘變下，還原蝦紅素含量增加至2.68 mg/L。而利用化學突變劑(NTG、EMS)方式突變菌株，添加0.1 mg/L NTG突變，還原蝦紅素含量達3.15 mg/L，而添加EMS 20~30 μL突變時，還原蝦紅素含量達到2.72 mg/L，顯示篩選之Paracoccus sp.可藉由不同的突變方式提高還原蝦紅素產量；篩選出之突變株反覆放大培養10次，還原蝦紅素產量可維持相當的穩定性。此外，3T3-L1脂肪細胞體外試驗，突變株在1~100 ug/mL濃度下，均具有抑制脂肪堆積的效果。

In this study two marine bacterial strains producing astaxanthin were acquired by screening, and the astaxanthin was extracted and analyzed. Those marine bacterial strains were identified by according to traditional biochemical and 16S rRNA as Paracoccus sp. (AS-14, AS-15). The optimal culture conditions of those strains could produce the highest astaxanthin content (0.2~0.71 mg/L) when incubated in Marine broth (MB) containing 0.25% sucrose and 0.5% fructose were the suitable carbon source. We studied their mutant conditions, including mutagenesis with microwave, UV, NTG, EMS mutagen and compound mutagenesis, were employed to increase the astaxanthin biosynthesis amount as well (2.68~3.15 mg/L), and were stabilized on transferred 10 times. Furthermore, showed effects on adipocytic differentiation of 3T3-L1 cells were examined, indicate that those mutant strains inhibited lipid accumulation at a concentration of 1~100 μg/mL.