農業部-水產試驗所全球資訊網農業部-水產試驗所全球資訊網

自由基在生物體內具有正反之雙重的生理意義， (Valko et al., 2006; Valko etal., 2007)，然而生物體內若產生過多的自由基，將造成氧化與抗氧化的失衡而形成氧化壓力 (oxidative stress)，並引發自由基之連鎖反應，產生氧化相關之疾病(Droge, 2002; Galli et al., 2005)或老化 (aging) (Galli et al., 2005;Martin and Grotewiel, 2006; Wong et al., 2006)。近年來研究發現，動植物性蛋白質或海藻之醣類可藉由水解作用，產生具有調節身體機能的活性胜肽與寡醣，而這些胜肽與寡醣因其結構、組成或聚合度之不同，已被證實具有抗氧化、延緩老化、降低血壓、免疫調節及降低膽固醇等多項生理功能 (黃與廖，2003；Wei and Chiang, 2009) 。因此本研究擬以酵素法水解魷魚皮或石蓴，以產製具有機能性之胜肽與海藻寡醣並組合成水產機能複方，探討其食用安全性、功效性及在氧化壓力下對神經細胞之保護作用，以期作為延緩老化用保健食品研發之參考。

ROS (related oxygen species) are known to play a dual role in biological systems, since they can be either harmful or beneficial to living systems (Valko et al., 2006; Valko et al., 2007). However, overproduction of ROS results in oxidative stress, a deleterious process that can be a important mediator of damage to cell structures, including lipids and membranes, proteins, and DNA. These changes and subsequent oxidative stress-induced oxidative damage to various biological molecules have been proposed as important factors in the aging and age-dependent diseases of humans and animals. (Galli et al., 2005; Martin and Grotewiel, 2006; Wong et al., 2006).Bioactive peptides and algae oligosaccharide from animal and plant proteins or seaweeds have been shown to exhibit a variety of functional properties, including antioxidant,anti-aging, angiotensin Iconverting enzyme inhibitory and immunomodulatory activities (Hung andLiao, 2003;Wei and Chiang, 2009).The aims of this study evaluate the anti-aging properties of marine functional formula with squid skin peptides and ulvan oligosaccharide, prepared from enzymatic hydrolysate
of squid skin and Ulva lactuca. And then regard the edible safety,efficiency and protected effect on neuroblastoma cell (SHSY5Y) under oxidative stress.