This study try to explore the suitable methods for induced spawning of the japanese eel (Anguilla japonica). Artificially induced sexual maturity of the eel used homogenized salmon pituitary mixed gonadotropin injection and mature female eels induced spawning used DHP injected. The results showed , we can decide the degree of gonadal development and the appropriate time for induced spawning of the eel by observing the color change of pectoral fin and urogenital opening , respectively.