In the recent years, the seed production of grouper is often hampered by high mortality rate due to infection of virus. To prevent this, application of immunomodulators in grouper larviculture is a potential strategy. Thus, herein we try to find some useful immunomodulators, which do enhance the immune activity of grouper juveniles, and to establish an analytic platform for the measurements of immune indicators. The immunomodulators will supplemented into the feeds and their effects affecting the immunity will be analyzed.