Seaweed is not only full with nitrous components including vitamins, minerals and dietary fiber, but also carries with bio-active substances. Those bio-active materials are potentially high values because of their functionalities comprising oxidation inhibitory, microbial resistance, cholesterol reduction, dietary fiber enrichment, immunity enhancement and body pH regulatory. Ulva and Enteromorpha are common green algae in Penghu, and usually apply in seaweed crackers production. They are underutilization. The proximal analyses and bioactivities assessments of the major green algae, Ulva and Enteromorpha, in Penghu are examined in this study. The samples are treated with enzymatic hydrolysis and solvent extraction, respectively, prior to bioactivity evaluation including antioxidative, bacterial inhibitory, and tyrosinase inhibitory activity tests. The higher bioactive effective samples are selected for complex skin care products formulation to increase the utilization and economic value of algae.