This study aims to selection the algae species/strains of Eustigmatophyceae for outdoor mass culture by algal physiological criteria, including response to diurnal fluctuations of temperature, resistance to photo inhibition, amount of dark respiration, and sensitivity to osmotic stress. And using the 1-L, 30-L and 40-L culture vessel to find the optimum culture condition for biomass and lipid production in nutrient sufficient and deprived media. Then the experiments of two-phase strategy will be carried out outdoor to test 3 types＆apos; culture systems for enhancement the biomass and lipid productivity. Three culture systems include annular reactors, raceway and plate reactor. In addition, the floating and flocculation system for harvesting of the microalgae will be developed to cost- down the dewatering process for biodiesel production.