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水產種苗團隊-水產種原分子生物技術輔助育種平台之建立

  • 日期:100-05-06
  • 計畫編號:98農科-1.1.2-水-A1
  • 年度:2009
  • 主持人:曾福生

為了解雜交優勢,利用不同來源的雜交仔代的表現分析九孔的成長特性及紅色吳郭魚的改良效果:(1)九孔,三種不同來源的雜交仔代,strain 1:由台灣養殖的雌貝與來自日本野生雄貝所產的子代;strain 2:台灣養殖貝所產子代和strain 3:台灣野生雌貝與養殖的雄貝所產的子代,成長性狀的表現,從3月齡開始經8個月的飼育比較,strain 1起始殼長22.13±5.7mm至10月齡36.26±5.77mm;strain 2,14.17±3.9mm至33.06±4.13mm;strain 3則13.25±2.9mm至31.73±3.54mm,以strain 1表現最佳而strain 2最差。在存活率方面,經過8個月的飼養,strain 1存活率97%,strain 2為83%,而strain 3則為98%。成長性狀及存活率在雜交的組合中以strain 1最佳,strain 3次之,結果顯示以親緣關係愈遠成長性狀及存活率的表現愈佳。(2)吳郭魚,以建立之紅色吳郭魚TsR品系和來自業者之吳郭魚CP1品系,二個吳郭魚的品系配對,正交:TsR♀xCP1♂和反交:TsR♂xCP1♀,自交組則分為TsR inbred,和CP1 inbred。4種不同的組合,經由形質測量及統計的結果顯示,在體高性狀表現方面,TsR與CP1兩者有顯著差異(p<0.01),正反交組合(ct)的體高表現都有顯著的改善接近或些微的高於CP1品系,體長表現則無明顯差異,顯示TsR品系體型屬修長型而CP1品系屬圓高型。以基因標誌群集分析4種不同的組合,當遺傳距離0.26時,可單獨將TsR品系分離出來,但無法區別CP1品系和正反交(TsR♀xCP1♂和TsR♂xCP1♀) (ct)。

研究報告摘要(英)


In order to understand hybrid vigor, we utilized progeny of from different sources crossing, analyzed the characteristics of growth small abalone, Haliotis diversicolor, and improvement of body shape in red tilapia, Oreochromis spp. : (1) Small abalone, hybrids of small abalone from three different sources mated, strain 1: hybrid crossing female farmed from Taiwan with the wild male from Japan; strain 2: inbred from farmed of Taiwan and strain 3 crossing wild female with farmed male all from Taiwan. We compared the shell length for 8 months since 3-months-old. Strain 1, the initial shell length 22.13± 5.7mm to the final shell length 36.26± 5.77mm; Strain 2, 14.17± 3.9mm to 33.06± 4.13mm and Strain 3 13.25±2.9mm to 31.73± 3.54mm. In survival rate, across 8 months, strain 1 is 97%, strain 2 is 83% and strain 3 is 98%. The characteristics of growth and survival rate is best with strain 1, strain 3 takes second place, strain 2 is the worst, these results showed that the far genetic relationship, the best performance on growth and survival rate. (2) Red tilapia, crossing red tilapia TsR strain with CP1 tilapia strain introduced from commercial strain, via line breeding, 4 different mating: forward hybrid (TsR♀xCP1♂)and reverse hybrid (TsR♂xCP1♀), inbreeding: TsR inbred and CP1 inbred, We analyzed the measurement of morphometric characteristics and statistics, showed that there were significantly (p<0.01) between TsR and CP1 strain on the body length and height. TsR body shape was a slender and CP1 belongs to the round high. Utilizing RAPD-PCR and Un-weighted Pair Group Method Analysis (UGPMA), when the genetic distance 0.26, we can isolate TsR inbred from reciprocally hybrid (TsR♀xCP1♂, TsR♂xCP1♀)and CP1 inbred.