The objectives of this project are to investigate the status of demersal fish resources in the northern waters off Taiwan, and provide a reasonable fishery management for sustainable utilization of fishery resources. The investigation on species composition in the northern waters of Taiwan was conducted by R/V Fisheries Research I with 38 hauls in 2 cruises. All specimens were recorded and including 79 species of 52 families of fishes, 13 species of 4 families of shrimps, 16 species of 6 families of crabs, and 3 species of 3 family of cephalopod. The 16 dominant economical species were Platyrhina sinensis, Galeus sauteri, and Uroteuthis (P.) edulis and so on. Our preliminary result of the population genetic structure analysis of benthic fish by mitochondrial partial sequence revealed that Priacanthus macracanthus off Taiwan can be divided into to clades, but mixed by Da-Xi group and Dong-Kong group, showed the metapopulation may present between these two groups. The D-loop primers design for Aristeus virilis was finished, and still need to increase PCR reaction sample size. Age and growth of Sphyraena japonica were examined in this study. The size at age estimated from the otoliths were 312-354 mm FL at 213-259 ages (days).