In this study, We found Japanese eel brookstocks cultured in freshwater for years can be induced feeding after seawater acclimation. The weight of those Japanese eel brookstocks is increased. After analysis of gonad development, The higher GSI(about 1.462±0.103%) of Japanese eel brookstocks stocked in seawater than in freshwater. The oocytes diameter of Japanese eel brookstocks stocked in seawater is also larger. We also try to induce mature of Japanese eel by using the technique of sex-hormone pellets implantation. The result shows the BWI(body weight index) of the female eels increased(about 103.44±1.103%) after two weeks of implantation of androgen pellet. And the GSI (about 4.85%) and oocytes(about 336±23.32um) diameter also increased after four weeks of implantation of androgen pellet. On the other hand, no significant difference of silvering situation and gonad development was found in the female eels with implantation of LHRHa pellet.