This plan conducted the masculinization of cobia fingerlings by the methyltesterone (MT) administration and cultivated to sex mature, then proceeded artificial insemination with the normal mature female. At present, we have cultured 5 batch of cobia progeny(6 moths old) and identify the sex type by gonadal histology. The female proportion were 25%, 34%, 60%, 23% and 34%, respectively. From result, we estimated that male breeders were normale male, not belong to neomale. We masculined another batch of cobia fingerlings for studying this problem. After masculining all cobia, stoped the oral administration of MT feed and conducted the following gonad development by gonadal histology. From result, although the gonad had grown to testis, also had the male function(spermiation), but as long as stoped feed MT, the testis still reversed to ovary. In reversing process, we can found the intersex stage in cobia gonad. This is an interesting and difficult topic to maintain the male fuction of cobia neomale for a long time and spawn with normal female to produce all-female progeny.