This study will be conducted to investigate the use of fisheries byproducts to culture live feed of fish fry. The optimal autolysis condition of tilapia by-product is pH 11, 50° C, buffer / substrate 2, hydrolysis time 24 hours, and the degree of hydrolysis (DH) is 18.84%. Following established the optimal autolysis condition, we compared the DHs with three different commercial proteases, the highest DH can reach 41.87%. The character of fish protein hydrolysate (FPH) by protease hydrolysis is grey-white, emulsified and no fishy odor. We also compared our FPH with commercial condensed fish soluble (CFS) and commercial tilapia FPH, the results showed that our FPH has highest concentration of α-NH 2 , indicated the efficiency of hydrolysis is better than commercial methods. We analyzed the proximate composition before and after hydrolysis, the crude protein increased from 36.30 to 48.10 %, and the ash content decreased from 35.84 to 18.93 %. Compared the amino acids composition with anchovy fish meal, arginine and isoleucine are relatively low, but glycine, proline and glutamic acid are relatively high. In the trail of culturing photosynthetic bacteria, the culture efficiency of FPH is higher than yeast extract, only 1/10 amount of FPH has similar effect to yeast extract on culturing photosynthetic bacteria.