In this study, We use androgen pellets implantation pre-treatment to improve the gonad development of Japanese eel brookstock, and follow up with traditional hypophysation-induced artificial maturation. This combine can make the brookstock to final maturation. The time using this combine method to reach final maturation (4 weeks) is less than only using the traditional hypophysation-induced artificial maturation(8-12 weeks).The less handling can decrease the intrusion of the routine injection. And we also found the male eel can spermiation with short time (4-6 week) by using androgen pellets implantation. The spermiation can last two weeks, and the sperm can be active by seawater and survived 30 minutes.