This project is to develop a flavor fish seasoning by the low-utilization aquatic resources and by-catch. After screens the suitable fermentation strain, we further to explore the optimum fermenting conditions , use Eurotium pesudoglaucum (No. E) to ferment fish meat, and use Aspergillus sp. (No. G) to ferment shiokoji, that can get a better effect. In addition, the fish fillet treated with shiokoji were used to analyze with the texture analyzer. The chewiness and firmness in the control group (0.5-1.5% salt treatment) were higher than those in the shiokoji group (5-15 %). While in the elasticity testing, the shiokoji group had a higher value. On the other hand, the peptides concentration of the products fermented with E or G were not significantly different (43 mg / mL after 14 days), but to 28 days the peptides content fermented with E strain was up to 50mg/ml. By sensory evaluation, the products fermented by E strain had significant difference in flavor , aroma and appearance that better than G strain, so use the E strain to ferment Shiokara seasoning should help enhance the product flavor.