Seahorse, Hippocampus a marine teleost fish, is one of the most famous and expensive materials of traditional Chinese medicine. There has always been that \\\\"the North ginseng, South hippocampus,\\\\" it said. Hence, hippocampus extract may has many physiologically active substances, it is worth to study. Penghu Marine biology Research Center was established hippocampus numerous farming techniques and technology transfer a number of manufacturers. It is believe that production of hippocampus and industrial development can be expected. This study was intends to use the artificial breeding of the hippocampus including Hippocampus kuda and Hippocampus procerus etc. After extracts of hippocampus, physiologically actives were measured, including antioxidant effects (DPPH and ABTS radical scavenging effect, Chelating ability and Reducing power), Ames test, anti-inflammatory (reduce NO and TNF-α inhibition), promoting hormones effect. We hoping to use this physiologically active substances could develop a new material or maintenance of health food.