Hybridization was carried out between kelp grouper (Epheluspine bruneus ,♀) and giant grouper (E. lanceolatus,♂), to study the embryonic development, growth and tolerance in low temperature. The results showed that the hybrid fertilized egg were round, spherical and ovum diameter of 0.875 ± 0.025 mm. There was an oil drop with a diameter about 0.225± 0.015 mm. The fertilized eggs was incubated at 25.5 ~ 28.5 ℃, salinity 33, pH 8.2 and hatching were observed at 25 h 30 min after fertilization. The growth performance of the hybrid larvae and juvenile were showed no significant difference compare to kelp grouper, and the hybrid adult was higher than the kelp grouper in the later stage of the grow out. The tolerance in low temperature of hybrid was showed the same characteristics as the kelp grouper with the low temperature resistance of 8.5 ℃.