Seaweed is often regarded as a “longevity delicacy”, because it is enriched in critical nutrients and minerals, which is thought to be beneficial to humans in physiological terms. It has been reported that extract from Botryocladia leptopoda, a kind of red alga, has strong antioxidant activity. We therefore propose here to extensively characterize the polyphenols containing in B. leptopoda for its potential activity in wound healing. We extract the polyphenol compounds from B. leptopoda and its residue in different procedures : EtOH-60; EtOH-Freezedry; Alkaline-EtOH; EtOH-70; and H2O sterilization. And the extraction product from EtOH-Freeze-dry contains much more polyphenols, the extraction product from Alkaline-EtOH taked a seconed place. Polyphenols from B. leptopoda can work as anti-oxidation in the 3 kind of index: reducing power, DPPH radical scavenging ability and ferrous ion chelating ability, but it is less effective in reducing power. In anti-inflammatory activity evaluation, the IL-6 variations in EtOH-Freeze- dry and Alkaline-EtOH are -2189% and -1673%, showing that these products play as a regulatory role in inflammatory cells. In the other ways, the concentration of transforming growth factor-1beta (TGF-1β) rise in both EtOH-Freeze-dry (45.40%) and Alkaline-EtOH (51.43%) groups, means the extracts has the potential for tissue repair. Add B. leptopoda extractions from EtOH-Freeze-dry and Alkaline-EtOH in the culture medium of mouse fibroblast cells L929, before add H2O2 .We can observe the concentration of execute superoxide dismutase (SODs) only increase when EtOH-Freeze-dry product exists. Obviously it has possibility of redox reducing superoxide(O2 - ), which can remove excessive free radical. Our long-term goal is to promote and maximize the utilities and the economic values of algae, thereby furthering the establishment of a vibrant algal industry in Taiwan.