Tilapia and bass are both important species for aquaculture in Taiwan. By the conventional culture, it emphasized and aimed at increasing the production volumes of the unit and increasing the culture density and fast-growing species to obtain the high production capacity. However, it was often neglected about the stability of the culture environment and the stress on the farmed species as well as the ecological balance of the surroundings. Especially, the temperature and water quality changes caused the stress which induced the disease outbreak due to the recent climate changes. The conventional solution is using the drugs to control the diseases and decrease the mortality, but the anti-biotic compounds cause some other problems like drug resistance, food security, water contamination, and cost. Therefore, it is essential to develop the technology instead of the anti-biotics for controlling the diseases. Recently, it is getting remarkably highlighted to treat the farmed tilapia and perches with the probiotics. The large-scaled feed manufacturers are not able to easily modify the production process to add the probiotics, because the microbial characteristics and adding process such as conservation and expiry dates are different. The project aims at the application of feed processing package with multiple probiotics. In 2022, we cooperated with a feed mill to commercially produce 74 tons of tilapia health feed containing probiotics Bacillus pumilus D5 and Leuconostoc mesenteroides B4 containing dextran. The results of the demonstration field showed that the use of tilapia health feed could effectively increase the average body weight and specific growth ratio under higher density feeding. The feeding rates were increased by 8.75%-14.5%, and the feed efficiencies were increased from 0.59 to 0.92. The medication cycles were reduced by 23-55%, and the relative ratios of total dosage/feed amounts were reduced by 40-86%. The incomes per unit area were increased by 1.6-2 times. In this year, we would build the demonstration field to verify perch health feed, increasing growth rate, survival rate and disease resistance.