Tilapia is one of important export seafood. Unapproved drugs administered to culturedfish pose a potential human health hazard and must be prohibited. The growing trendtowards world trade liberalization has spurred pronounced in interest in thedevelopment of more reliable and efficient quality assurance systems. While this trend isbasically oriented to ensure better protection of the consumer, it is also aimed atdeveloping a more homogeneous base for the establishment of commercial agreementsamong countries, and at the same time, improve the international framework to solvefood safety and product wholesomeness issues. This trend has been particularlyimportant for the production and marketing of fishery products, resulting in variousinternational agreements to adopt the basic HACCP principle as a common regulatorybase. A liquid chromatography mass spectrometry method for the determination ofmicro-level residues of aquaculture drugs and unapproved drugs in tilapia will beestablished to screen the hazards in on-farm tilapia for routine monitor in this study. Thesafety and sanitation of fish production will be control in aquaculture farm and toestablish the monitoring procedures for drug residues and sanitation control in culturedtilapia.