The achievements of the project this year are (1) screening microsatellite DNA markers of tilapia and small abalone; (2) setting up the second generation of TsB and TsR tilapia; (3) analyzing the relationship between the gene markers and characters of tilapia; (4) clustering analysis for the growth rate of small abalone; (5) setting up the gene marker plasmid library of small abalone, and (6) developing the model of fast detection kit for the pathogens of small abalone. In tilapia, 30 samples, three month-old, randomly sampled from generation of F1, F2 and F3 each full-sibling, measuring the body length and weight. The body length and weight of F1 were 76.42±8.51mm and 7.19±3.29g; F2：73.59±18.55mm and 7.19±3.29g; F3：70.14±4.32mm and 4.05±1.79g. Detected by t-test, both body length and weight were no difference between F1 and F2. The body length was no difference between F2 and F3, however, the body weight was difference (p<0.01). Both body length and weight were difference between F1 and F3 (p<0.005). Microsatellite primer fingerprinting was carried out to screening the color-associated DNA sequence for color-associated in tilapia. 20 primers, from NCBI, were used for PCR. Two of these primers (Red-1 & Red-13) produced specific marker (200 bp and 1300 bp) only found in tested red color tilapia of TsR line but none found in black color tilapia of TsB line. This result showed that the color-associated DNA marker will be effectively selected color marker for tilapia breeding in the future. In small abalone, utilizing RAPD technique was applied to assess the genetic variation of small abalone, Haliotis diversicolor, (1) there were five farmer strains, two of them were from the county of Taitung (TC1 and TC2) and the others from the county of Yilan (YC1, YC2 and YC3), (2) wild abalone were from the coast of Shueilian-Jichi (HW1) and Yanliao (HW2) of Hualian County, respectively. Six clear and reproducible random primers (p3, p5, p7, p8, p9 and p12) were used and 61 bands were generated. The results showed that in farmer strains the observed number of alleles (na) was 1.8033±0.4008~1.8852±0.3214; effective number of alleles (ne) was 1.5023±0.3524~1.6469±0.3423; gene diversity (h) was 0.2927±0.1830~0.3599±0.1638; Shannon's Information index (I) was 0.4360±0.2522~0.5233±0.2210, the number of polymorphic loci (nL) was 49~54, and the percentage of polymorphic loci (pL) was 80.33%~88.52%, besides Y3 population. In wild abalone the (na) was 1.8689±0.3404~1.9508±0.2180; (ne) was 1.6677±0.3456~1.7397±0.2846; (h) was 0.3659±0.1717~0.4048±0.1298; (I) was 0.5268±0.2356~0.5823±0.1703, (nL) was 53~58, and (pL) was 86.89%~95.08%. Based on the results and through un-weighted pair group method analysis (UPGMA), these small abalone can be divided into three subgroups: Taitung, Haulian, and Yilan. Compared to the farmer strains, the wild abalone from Haulian displayed a greater genetic variation, polymorphism and number of alleles. In addition, the prototype of fast detection kit of Vibrio alginolyticus has been developed. We expect the achievements of this project can assist the industry of tilapia and small abalone aquaculture in stock selection and artificial breeding, can keep away from the abuse or misuse of medicines, and to male aquaculture in Taiwan to be a sustainable environment-friendly industry.