Oyster shell was regarded as fishery waste for long time, therefore how to apply oyster shell which is one of the most important issues in recent year. In this study, we developed multiple applications of pearl layer peptide and burned power extracted from oyster shell. After burning, the total superficial area of burned oyster shell was larger than un-burned one and particle diameter was smaller. These physical properties could raise the absorbability of low molecular weight substance. About the application of burned oyster shell used in anti-bacterial, 0.25% burned oyster shell can inhibit the growth of Staphylococcus aureus, Escherichia coli, Listeria monocytogenes, Salmonella typhimuriun, Bcillus cereus and Micrococcus luteus. Since the anti-bacterial property of burned oyster shell, when vegetables and fish fillet after cleaning by burned oyster shell solution, the storage time could be elongated. And it also can reduce the total plate count of toothbrush and duster. Air conditioner filter and fiber containing burned oyster shell, which also have the ability of anti-bacterial and anti-mold. The pearl layer peptide could prevent the keratinocyte cell from UV injury, but there was no significant effect on cure for UV injury.