The carbon dioxide is added in microalgae culture to investigate the absorption of carbon dioxide on microalgae. The result shows that absorption rate of carbon dioxide is 1.2 ppm/hr. Moreover, the carbon dioxide is added into sea water, oyster shell powder mixture liquid, and alkali liquid. The oyster shell powder mixture liquid and alkali liquid react with carbon dioxide and produce calcium hydrogen carbonate of high solubility in water. It can offer microalgae culture with carbon source. To compare the absorbed capacity, the highest capacity is alkali liquid, than followed with oyster shell powder liquid, and sea water. The highest dissipation rate of carbon is sea water. The absorption rate of calcium hydrogen carbonate on microalgae is 1.3 ppm/hr. To analyze the lipid content of microalgae, the NIR method is being used. The precision lipid content of microalgae must under the moisture content of microalgae decreasing lower than 98 %. To compare the lipid productivity of low nitrogen and continuous culture method, the result shows that the continuous culture has higher lipid productivity than low nitrogen method.