The aim of the study is to investigate: (1). The annual changes in hemolymphatic parameters of the small abalone broodstock and in environmental variables. (2). The impacts of inducing spawning on hemolymphatic parameters of the abalone. In the first experiment, we found that the serum sugar, protein, total hemocyte number and immune enzymes in the abalone during the reproductive season, October to January, were lower than those during the non-reproductive season. Water temperature and gonadal maturation could play major roles in this change.
In the second experiment, mature adult abalone was induced to spawn by sun-exposure and thermal shock. They spawned in the end of the cooling. The results showed that sun-exposure and spawning significantly induced higher content of serum sugar but did not produce a significant impact on the serum protein, hemocyanin, and osmolarity. The activity of ALP did decrease after spawning, although those in the other 3 immune enzymes did not change significantly.