Farmed Caulerpa microphysa, so called grape algae in Penghu, will be dehydrated and pulverized for proximate compositions, heavy metals, free amino acid and fatty acids analyses. Dried samples will be hydrolyzed with Flavourzyme, Alcalase and Pepsin, respectively. Then, hydrolyzed samples will be spray dried, and analyzed for antioxidative, bacterial inhibitory, and Ames tests.
The results showed that grape algae heavy metals contains Al, Mn, Cu, As, Se, Cr, Cd and Pb, Fatty acids analyses contains Monounsaturated fatty acids 24.21%, polyunsaturated fatty acids 29.12%, saturated fatty acids 46.67% and ω-3 fatty acid 12.68%, Free amino acid including o-Phosphorserine, Taurine, L-Aspartic Acid, L-Threonine, L-Serine, L-Glutamic acid , Glycine, L-Alanine, L-Valine, L-Isoleucine, L-Leucine, L-Phenylalanine, Ethanolamine, and DL-plus allo-δ-Hydroxylysine.
In conclusion, the antioxidant capacity of Caulerpa microphysa extract by Pepsin digestion has better antioxidant activity than other treatment. To evaluate the antibacterial activity of Caulerpa microphysa extract by Lactobacillus fermentation, some of the extracts have antibacterial efficacy. In Ames test, Caulerpa microphysa extract had cytotoxicity and mutagenicity in S. typhimurium TA 98 and TA 100 systems. The data shows Caulerpa microphysa extract is the safety material of health care sources.
The objective of this study is determination of the most suitable extraction methodology for development the grape algae functional healthy products. Consequently, create high value and full utilization for farmed grape algae in Penghu.