The main purpose of this study, to construct the mode of selection and breeding to establish, strengthen Gentian grouper grow breeding operation platform, this year's performance goals for the continued excellent species of fish selection. The test results show that after selection, the fish (A1) average weight of 14.5kg body length 82cm, after elimination fish average weight of 10.4kg body length of 80 cm, with a significant difference in the patterns. Establish the giant grouper functional gene cloning, the successful cloning MEF2D of AKT2 TGF beta receptor and protein synthesis and muscle growth related genes, and the relative quantification of tissue distribution. The results showed that MEF2D has higher performance in the caudal fin, gill, muscle, spleen, and scales. AKT2, TGF beta receptor also had similar results, and finally in the genetic characteristics, above the initial establishment of the multi on microsatellite SSR primers screened, the batch with the growth advantage genotype fish only will last Keeper conservation, the microsatellite primers available for follow-up over the multi-batch analysis.