農業部-水產試驗所全球資訊網農業部-水產試驗所全球資訊網

本試驗以1對1自交配對方式，繁殖3對尼羅吳郭魚(N2)品系。此外，參考吳郭魚遺傳聯鎖圖譜，以微衛星方法分析LG1、LG3、LG14、LG15及LG23等區域之基因座多型性分析，檢測將近40對引子組，篩選到6個具鑑別力的基因座(UNH995、GM354、UNH898、UNH168、UNH155與UNH986)。利用該基因座的多型性，以3個基因座(UNH995、GM354及UNH898)可鑑別3個尼羅吳郭魚家系。另外，將6個微衛星基因座資訊登錄於水產生物種原資訊管理系統(http://agin.ph.tfrin.gov.tw/wfg/)，以建立吳郭魚遺傳資料庫。

Using single-pair mating, 3 Nile tilapia families (N2 strain) were produced. Besides, consulting the second-generation linkage map of the tilapia genome, we used microsatellite primer to analyze genetic pleomorphism in the linkage group 1(LG1), LG3, LG14, LG15 and LG23. After detecting about 40 locus, we selected 6 locus (UNH995, GM354, UNH898, UNH168, UNH155 and UNH986) which were pleomorphism. Three (UNH995, GM354 and UNH898) of 6 locus could be used to distinguish 3 Nile tilapia families. Furthermore, we have kept a record of microsatellite locus information on website (Aquatic germplasm information network in Taiwan, http://agin.ph.tfrin.gov.tw/wfg/) for establishing genetic library of tilapia.