Six experimental diets were formulated to be iso-nitrogenous (500 g/kg), iso-lipidic (70 g/kg), varying only in their lipid sources (cod liver oil (F), linseed oil (L), sufflower (S) oil, canol oil (C) or olive oil (O). The lipid mixture contain containing 50 % cod liver oil and other vegetable oil source, and designated as L1 (50% L)、LC (25% L + 25% C), LO (25% L + 25% O), S1 (50% S), SC (25% S + 25% C) and SO (25% S + 25% O). Feed were hand fed to homogenous groups of 15 Epinephelus coioides juvenile (mean weight 6.8 ± 0.3 g) per tank in triplicate for 8 weeks, in a recirculation system. Whole-body proximate composition analysis was similar among all the treatments. The growth performance and feed efficiency in the LC and LO treatments were significantly (P < 0.05) higher than those in the other treatments respectively. Based on the results of this trial, suggesting that optimum ratio 18:3n-3 and 18:2n-6 produce better growth, moreover, monounsaturated fatty acid in the diet for E. coioides juvenile, to achieve good growth performance.