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豹鱠種魚培育及幼苗量產技術研發

  • 日期:104-03-10
  • 計畫編號:103農科-11.3.3-水-A1(3)
  • 年度:2014
  • 領域:漁業科技研發領域
  • 主持人:許鐘鋼
  • 研究人員:林金榮、陳岳川、莊成意、蘇勰忠、黃金峰、陳文展

本年(103)度將豹鱠種魚分成野生及人工養殖兩組(均未經激素誘導產卵)進行交配產卵。4月14日進行野生及人工豹鱠種魚之室外移地養殖,5月28日移入室內產卵池。6月4日發現交配產卵,產卵期從6月4日至8月27日,產卵期達85日,產卵日80日。總產卵量92,742g(18,548.4萬粒),其中上浮卵67,618g(72.91%;13,523.6萬粒),下沉卵25,124g(27.09%;5,024.8萬粒)。平均日產卵量1,159±1,118g(231.9萬粒),日產卵量最高4,482g(896.4萬粒)。
野生種魚產卵期從6月4日至8月27日,產卵期85日,產卵日77日。共產卵86,517g(17,303.4萬粒),上浮卵65,425g(75.62%;13,085.0萬粒);下沉卵21,092g(24.38%;4,218.4萬粒)。平均日產卵量1,124±1,025g(224.8萬粒),日產卵量最高3,951g(790.2萬粒)。人工種魚產卵期從6月6日至8月24日,產卵期80日,產卵日40日。共產卵6,225g(1,245.0萬粒),上浮卵2,193g(35.23%;438.6萬粒);下沉卵4,032g(64.77%,806.4萬粒)。平均日產卵量178±172g(35.6萬粒),日產卵量最高758g(151.6萬粒)。
產卵期間種魚養殖水溫範圍24.9-30.8℃。豹鱠幼苗培育約1,968尾,完成馴餌1,700尾。
收集豹鱠受精卵、魚苗進行神經壞死及虹彩病毒之檢測。神經壞死病毒RT-PCR檢測結果為:受精卵呈陽性反應為0%(0/130)。魚苗呈陽性反應為0%(0/10)。虹彩病毒PCR檢測結果為:受精卵呈陽性反應為0%(0/130)。魚苗呈陽性反應為0%(0/10)。

研究報告摘要(英)


There were two treatments in the broodstock of Plectropomus leopardus, wild broodstock and cultured broodstock(with habitat moving). The Spawning period of wild broodstock was from June 4 to August 27 and lasted 85 days, including 77 spawning days. The total egg weight was 86,517g(17,303.4×104 eggs),including the buoyant eggs(65,425g; 13,085.0×104 eggs; 75.62%)and the sinking eggs(21,092g; 4,218.4×104 eggs; 24.38%). Mean egg weight was 1,124±1,025g(224.8×104 eggs),and the highest number of daily spawning was 3,951g(790.2×104 eggs).The Spawning period of cultured broodstock was from June 6 to August 24 and lasted 80 days, including 40 spawning days. The total egg weight was 6,225g(1,245.0×104 eggs),including the buoyant eggs(2,193g; 438.6×104 eggs; 35.23%) and the sinking eggs (4,032g; 2, 806.4×104 eggs; 64.77%). Mean egg weight was 178±172g(35.6×104 eggs),and the highest number of daily spawning was 758g(151.6×104 eggs).
During the period, the water temperature was 24.9-30.8℃.Fertilized eggs were hatching in 10 mt FRP tank fed with micro-zooplankton. There were 1,700 larvae till alive and the total body length was 15-18cm.
We collected fertilized egg and the larvae samples of Plectropomus leopardus to detect for NNV and GIV. The result of NNV RT-PCR test of fertilized egg showed that the ratio of positive was 0%(0/130)and the juvenile was 0%(0/10). The result of GIV PCR test of fertilized egg showed that the ratio of positive was 0%(0/130)and the juvenile was 0%(0/10).