A red algae species called Asparagopsis taxiformis inhibit the waters of Taiwan's Pingtung. In recent years, the species has been discovered to effectively mitigate methane emissions from ruminant animals, but a commercial cultivation technique has yet been established. Currently, the main method of acquiring A. taxiformis is through the collection of wild algae, resulting in unstable production and quality due to seasonal variations. This study investigated the cultivation conditions and found that the algae's growth was significantly better at water temperatures of 24-30℃ compared to 15-21℃. No significant differences were observed within the higher temperature range, but 24℃ was the optimal temperature. In terms of light intensity, the cultivation results showed a significant improvement at 5,000-10,000 lux compared to 2,500 lux, and no significant differences were observed within the high intensity group. Regarding light wavelengths, full spectrum (white light) showed significant superiority in growth compared to red, blue, and green light. No significant differences were observed among the different photoperiods. Furthermore, molecular biology identification confirmed that the A. taxiformis collected from Hengchun Township and Liuqiu Township in Pingtung County as well as Chenggong Township in Taitung County were all of the same species. Going forward, further investigation on the effects of salinity and nutrient formulations on the cultivation of this algae could establish cultivation techniques for A. taxiformis, providing valuable references for academia and various industries.