農業部-水產試驗所全球資訊網農業部-水產試驗所全球資訊網

為提供給養殖業者作為常用藥物施藥參考的依據，本試驗以六種化學治療劑孔雀綠、甲基藍、硫酸銅、福馬林、高錳酸鉀及美舒添，探討在20℃ 及30℃ 水溫中對青魚之急性毒及半致死濃度 (LC50)，並測試一般建議使用濃度及藥浴時間是否適用於青魚。
結果顯示，六種藥物對青魚96小時之LC50值，在20℃ 時分別為孔雀綠0.117 ppm、甲基藍 35.542 ppm、硫酸銅1.183 ppm、福馬林85.058 ppm、高錳酸鉀3.451 ppm及美舒添14.612 ppm；在30℃ 時分別為孔雀綠0.114 ppm、甲基藍18.451 ppm、硫酸銅0.976 ppm、福馬林84.127 ppm、高錳酸鉀 3.105 ppm及美舒添7.226 ppm。由LC50值顯示毒性以孔雀綠最強，其次依序為硫酸銅、高錳酸鉀、美舒添、甲基藍、福馬林。在30℃ 水中毒性都有昇高趨勢，其中更以甲基藍及美舒添最明顯。另外，一般建議以孔雀綠0.15 ppm及高錳酸鉀 3～5 ppm 作長時間藥浴，並不適用於青魚。
關鍵詞：青魚，化學治療劑，半致死濃度

The aim of this study is to investigate the median lethal concentrations (LC50s) of six practical chemotherapeutic agents on black carp Mylopharyngodon piceus under two ambient temperatures (20℃ and 30℃). The suitabilities of commonly recommended concentrations of agents are also examined for black carp. When the water temperature was 20℃, after 96 h of exposure, the LC50s of malachite green, methylene blue, copper sulfate, formalin, potassium permanganate, and masoten were 0.117, 35.542, 1.183, 85.058, 3.451, and 14.612 ppm, respectively. Furthermore, agents appeared stronger toxicities when the temperature was 30℃, and the LC50s were 0.114, 18.451, 0.976, 84.127, 3.105, and 7.226 ppm, respectively. The relative strength of agents is in the following order: malachite green, copper sulfate, potassium permanganate, masoten, methylene blue, and formalin. The results also showed that the commonly recommended concentrations of malachite green (0.15 ppm) and potassium permanganate (3-5 ppm) are not suitable to black carp for long-term exposure.
Key words：Black carp Mylopharyngodon piceus, Practical chemotherapeutic agents, Median lethal concentration