This study was conducted to evaluate the effect of artificial diets containing different lipid levels on ovarian maturation, spawning conditions, and survival of unilateral eyestalk-ablated female broodstock of Litopenaeus vannamei. Diets with 4 lipid levels of 0%, 1%, 3%, and 5% were used in this study. The ratio of cod liver oil to corn oil as the lipid source was 2:1. The protein sources were fishmeal and squid meal. In terms of the maturation rate, number of eggs spent, spawning rate, and survival, the best results were obtained in the group of females fed a diet containing 1% lipids (11.4% crude lipids, 52.74% crude protein, and 105 mg protein/kcal P/E ratio), while worse results were obtained in groups fed diets containing 3% or 5% lipids.