The proximate composition, amino acid composition, and fatty acid composition of cultured and wild milkfish (Chanos chanos) were analyzed and compared. The condition factor of cultured fish was significantly (p < 0.05) higher than that of wild fish. The crude fat content in cultured fish was higher, but the moisture, crude protein, and ash contents were lower than those of wild fish. The amino acid composition showed that both cultured and wild fish were rich in aspartic acid, glutamic acid, leucine, histidine, lysine, and arginine. The fatty acid composition of lipids differed between cultured and wild fish. Cultured fish had higher levels of 18:1 and 18:2 fatty acids, but wild fish had higher levels of 16:0, 18:0, 20:4, 20:5, and 22:6 fatty acids. The cultured fish had a higher amount of monoenes, but were lower in saturated fatty acids. Polyenes showed no significant differences between cultured and wild fish.