農業部-水產試驗所全球資訊網農業部-水產試驗所全球資訊網

本研究探討真鎖管之水溶性蛋白質酵素水解物之生理活性。真鎖管之水溶性蛋白質分別以未添加酵素及添加papain、bromelin、protease Type XIV、protease Type XXIII等不同蛋白質分解酵素，在37 ℃下，經0、6、12、18、24 hr作用後，獲得水解物。然後進行各該水解物之Trolox抗氧化當量 (TEAC)、還原力、清除DPPH (α, α-diphanyl-β-picrylhydrazyl) 自由基及螯合銅離子等之效果評估。實驗結果發現，以0.02% 的protease Type XIV進行蛋白質水解18 hr後，TEAC值較高，其值為1.14 ± 0.01 mM。以0.02% 的papain進行水解12 hr後，清除DPPH自由基能力與還原力皆較其他條件為佳。其中，清除DPPH自由基的能力為80.78 ± 0.69%，而還原力值為0.27 ± 0.01。至於螯合銅離子部份，則是以利用protease Type XIV酵素水解12 hr為最佳，其值為12.76 ± 0.81%。

The objectives of this research was to study the functional activity peptides from neritic squid (Loligo edulis) sarcoplasmic protein hydrolysate. The neritic squid sarcoplasmic protein was hydrolyzed with papain, bromelin, protease Type XIV, protease Type XXIII at 37 oC for 0, 6, 12, 18, 24 hr and companied with the control group. The antioxidative activities of protein hydrolysates were measured by four methods inculding the Trolox equivalent antioxidant capacity, scavenging effect of DPPH (α, α-diphanyl-β-picrylhydrazyl) radical, reducing power and chelating Cu2+ effect. The hydrolysate obtained by treating with 0.02% protease Type XIV for 18 hr, showed the better Trolox equivalent antioxidant capacity (value = 1.14 ± 0.01mM) than others . As treating with 0.02% papain for 12 hr, the scavenging effect of DPPH radical and reducing power reached the higher value, 80.78 ± 0.69% and 0.27 ± 0.01 respectively, than others conditions. The highest values of chelating Cu2+ effect, 12.76 ± 0.81% was obtained by treating with 0.02% protease Type XIV for 12 hr.