跳到主要內容區塊

九孔苗池之底棲矽藻的分離與鑑定

  • 出版日期:97-06-30
  • 標題title(英):
    Isolation and Identification of the Benthic Diatoms from Small Abalone (Haliotis diversicolor) Larva Rearing Pond
  • 作者:蘇惠美‧張銀戀‧鄭伊惠‧王淑欣‧陳紫媖
  • 作者auther(英):Huei-Meei Su, I-Lian Chang, Yi-Huei Cheng, Sui-Sin
  • 卷別:16
  • 期別:1
  • 頁碼:39-53

底棲矽藻是九孔育苗時不可或缺的餌料生物,餌藻的質與量,影響苗之生長與活存。傳統以附板上自然發生的藻膜,作為九孔苗的食物,其餌料品質難以控制。本研究自養殖池分離可能適合九孔苗之底棲矽藻,加以保種及鑑定,以供後續增殖條件及餌藻效益之探討。在解剖顯微鏡下利用毛細管分離法,挑出藻細胞,逐步純化後成為單株藻種。以洋菜斜面及液體繼代培養作種原保存,共分離12種藻株。分離之單株矽藻分別以光學顯微鏡及掃描式電子顯微鏡觀察。依據色素體、細胞形狀及細胞殼的微細構造等,鑑定種名。鑑定至種名的8藻株為:潛艇曲殼藻 (Achnanthes submarina)、巴拉曲殼藻 (Achnanthes parvula)、扁圓卵形藻多孔變種 (Cocconeis placentula var. euglypta)、馬氏舟形藻 (Navicula margalithii)、簡單雙眉藻 (Amphora exigua)、穀皮菱形藻 (Nitzschia palea)、反轉菱形藻 (Nitzschia lorenziana var. incerta) 及翼繭形藻 (Entomoneis alata);其他4藻株鑑定到屬,其中Achnanthes sp.一株及Seminavis spp. 三株。

摘要abstract(英)


Benthic diatoms are the principal food sources for postlarval abalone in hatcheries, and the survival and growth of postlarvae depend on species and abundance of the diatoms. However, the occurrence of diatoms grew on the plastic plates in traditional way is difficult to manage. This study aims to isolate and identify suitable diatoms for larviculture of small abalone. Unialgal cultures of diatoms were achieved, with the alga was isolated by pipette under the stereomicroscope. Twelve stock cultures were maintained in liquid medium or agar slant for a long-term storage. Diatoms were observed first under the light microscope and later by the scanning electron microscope. The morphology of plastids, cell shape and fine structure of silicate cell wall were used as the taxonomic characteristics. Eight diatoms species were identified as Achnanthes submarina, Achnanthes parvula, Cocconeis placentula var. euglypta, Navicula margalithii, Amphora exigua, Nitzschia lorenziana var. incerta, Nitzschia palea, Entomoneis alata. In addition, four diatoms were classified to genus as Achnanthes sp. and Seminavis spp.