農業部-水產試驗所全球資訊網農業部-水產試驗所全球資訊網

本研究探討飼料添加多醣 (β-1,3-1,6-glucan from Schizophyllum commune) 對海鱺免疫酵素活性之影響。平均體重50 ~ 80 g之海鱺投餵含0.5% 多醣之飼料與不含多醣之飼料60天，並於投餵飼料後之第0、1、 3、6、12、20、30、40、50與60天，進行超氧陰離子 (O₂^-)、超氧岐化酶 (SOD)、溶菌酶 (lysozyme) 之測定。結果顯示，海鱺餵飼添加多醣類飼料後，試驗魚之超氧陰離子與超氧岐化酶產生量及溶菌酶活性均較未添加組明顯地上升 (p < 0.001)，並分別於餵飼後之第12、20與24天達到最高點；但超氧陰離子與超氧岐化酶之產生量分別於投餵後之第30、40天開始降低，溶菌酶活性亦於第40天後出現減弱現象，而與未添加組無顯著差別 (p > 0.05)。從本研究的結果來看，使用多醣0.5%，餵食6天以上，即可明顯增強海鱺的免疫能力，但長時間連續投餵，反而使海鱺免疫系統的活化力會降低，因此多醣的使用必須配合適當的投餵時間。

The present study was conducted to investigate the effects of dietary -1,3-1,6-glucan (BG) from Schizophyllum commune on the innate immune response in cobia (Rachycentron canadum). Cobia (50-80 g) were fed a basal diet and supplemented with either 0 (control group) or 0.5% BG (glucan group) for 60 days. The superoxide anion (O₂^-), superoxide dismutase (SOD) concentration and lysozyme activity were analyzed at days 0, 1, 3, 6, 12, 20, 30, 40, 50 and 60. The results showed that the concentration of O2- and SOD and activity of lyszoyme in cobia at BG group was higher (p < 0.001) than those of the control group. They attained the highest levels at day 12, 20 and 24 after feeding, respectively. However, the concentration of O2- on day 30, SOD on day 40 and activity of lysozyme on day 40 of the fish at BG group, dropped back to the same levels as the control group (p > 0.05). The results in this study showed that oral administration of 0.5% BG for 6 days enhanced immunity of the cobia, but prolonged use of BG would not increase the immunity of the cobia. Care therefore must be taken to maximize its effectiveness with suitable period.