農業部-水產試驗所全球資訊網農業部-水產試驗所全球資訊網

將庫達海馬 (Hippocampus kuda) 區分為腹部與其他兩部分，分別以熱水及酒精萃取法處理，評估其抗氧化能力及對免疫細胞活性之影響。抗氧化能力是以四種體外分析法進行，包括清除DPPH自由基能力、SOD-like活性、還原力及螯合亞鐵離子能力，結果顯示40 mg/ml濃度下，兩種萃取法的腹部萃取物，除螯合亞鐵離子能力不顯著 (均低於9％) 外，三種抗氧化能力分析法的活性表現，均高於其它部分之萃取物。對免疫細胞活性影響之評估，是以HB4C5及J774.1二細胞株進行。腹部熱水萃取物 350 μg/ml濃度，能刺激HB4C5細胞增生活性達155％，IgM抗體分泌達140％；此外腹部酒精萃取物在130 μg/ml濃度下，亦能刺激細胞增生活性 (142％) 及IgM抗體分泌 (130％)。對於J774.1細胞活性的影響：腹部熱水萃取物之濃度與細胞增生活性呈現正相關，在700 μg/ml濃度下細胞增生活性達148％ 最大值，腹部酒精萃取物在75~520 μg/ml 濃度範圍間，對細胞增生活性則無顯著差異。

Spotted seahorses, Hippocampus kuda , were separated into two portions: belly and rest body portion. Antioxidative activity of hot-water and ethanol extract of Hippocampus kuda seahorse was evaluated using four different methods, including DPPH free radicals scavenging activity, SOD-like activity, reducing power and Fe+2 chelating activity, and immune activity was assessed by using HB4C5 and J774.1 cells. It was found that the antioxidative activities of belly extract were higher than the extract from the rest body portion, but the Fe+2 chelating activities were all lower than 9%. The HB4C5 cells showed the maximum relative activities of cell proliferation (155%) and IgM secretion (140%) at 350 μg/ml of hot-water belly extract. The ethanol belly extract at 130 μg/ml under could stimulate cell proliferation (142%) and IgM secretion (130%). At 700 μg/ml, the extract showed the maximum relative activities of cell proliferation (148%) of J774.1 cells.