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海馬熱水萃取物對於抗疲勞與免疫調節功能之評估

  • 出版日期:98-12-31
  • 標題title(英):
    Antifatigue and Immunomodulatory Effects of Hot Water Extracts of Seahorse
  • 作者:簡世勇‧洪郁嵐‧黃培安‧吳純衡
  • 作者auther(英):Shih-Yung Chien, Yu-Lan Hung, Pai-An Hwang and Chw
  • 卷別:17
  • 期別:2
  • 頁碼:97-106

海馬在亞洲地區廣泛地作為中藥材之用,中醫認為海馬之功效主在提升精力及抗疲勞。而本研究旨在利用動物模式瞭解傳統中藥-海馬之功能性,以作為中藥科學化之基礎數據。將養殖庫達海馬與野生棘海馬之熱水萃取物餵食小鼠,6週後進行抗疲勞與免疫調節之功能評估。抗疲勞功能評估為偵測小鼠游泳時間、血乳酸 (Serum lactate)、肌酸激酶 (Creatine kinase) 與尿素氮 (Blood urea nitrogen) 等生化值;免疫調節評估以偵測脾臟細胞增生能力 (spleen cells proliferation)、脾臟淋巴細胞亞群分佈 (Lymphocyte subpopulation of spleen cells)、自然殺手細胞活性 (Natural killer cell activity) 與吞噬細胞活性 (Phagocyte activity) 等指標。抗疲勞評估方面,給予小鼠養殖庫達海馬與野生棘海馬之熱水萃取物,皆能延長動物游泳時間及降低血中肌酸激酶與尿素氮的含量。而在免疫調節評估方面,給予小鼠上述2種海馬之熱水萃取物,則是對於四項免疫功能指標皆無造成任何影響。顯示養殖庫達海馬與野生棘海馬之熱水萃取物對於小鼠具有抗疲勞之功效,但對免疫調節則無功效表現。所以,養殖庫達海馬與野生棘海馬之熱水萃取物皆具有抗疲勞之功效性且無顯著差異,站在生態保育的角度上,養殖庫達海馬具有取代野生棘海馬之可行性。

摘要abstract(英)


The seahorse has been used extensively in Asia as a traditional medicine whose functions may be enhance energy and fatigue resistance. The effects of hot water extracts from cultured seahorse Spatiotemporal Variations of Abundance and Species Compositions of Larval Fish in Tapeng BayHung-Yen Hsieh1, Yi-Tien Su1, Wen-Tseng Lo2* and Long-Jing Wu11Coastal and Offshore Resource Research Center, Fisheries Research Institute2Institute of Marine Biotechnology and Resources, National Sun Yat-Sen UniversityABSTRACTThe objectives of this study were to investigate the species composition and distribution of larval fish in relation to water temperature, salinity and chlorophyll a in Tapeng Bay. There were 31 identified species of the larval fish that belonged to 29 genera and 16 families with the mean numerical abundance of 22.9 ± 40.7 ind./100m3 from June 1999 to December 2001. The seasonal change in numerical abundance of the larval fish was not coincident interannually. The six most dominant taxa were Omobranchus sp., Tridentiger obscurus, Pterogobius zonoleucus, blenniid sp., gobiid sp., and Entomacrodus lighti, with occupied 92% of the total abundance. Among these, the first two dominant taxa, Omobranchus sp. and T. obscurus contributed to 80% of the total count. In linear regression analysis, there was no significantly different between environmental factors and abundance, excepting a positive relation between Omobranchus sp. and water temperature (p < 0.01). In addition, the ANOVA test was showed that there was no significantly different between abundance and months and stations.Hippocampus kuda and wild seahorse H. enrinace on antifatigue and immune were compared. Two kinds of extracts were separately fed to mice for six weeks. The duration of swimming time, serum lactate, creatine kinase, blood urea nitrogen, spleen cells proliferation, lymphocyte subpopulation of spleen cells, natural killer cell activity and phagocyte activity on forced swimming stressed mice were analyzed. Both extracts significantly extended the swimming time and reduced creatine kinase and blood urea nitrogen in plasma. The spleen cells proliferation, lymphocyte subpopulation of spleen cells, natural killer cell activity and phagocyte activity of two extracts were no significantly different from control. Therefore, it suggested that the two extracts might have an effect on the antifatigue action.