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不同矽藻對九孔苗活存與成長的影響

  • 出版日期:99-12-30
  • 標題title(英):
    Effect of Diatoms on the Survival and Growth of Post-larval Abalone (Haliotis diversicolor)
  • 作者:蘇惠美‧張銀戀‧王淑欣‧謝隆聲‧陳紫媖‧何源
  • 作者auther(英):Huei-Meei Su et al.
  • 卷別:18
  • 期別:2
  • 頁碼:75-87

為解決九孔苗的餌藻問題,本研究以來自不同雌雄種貝生產的浮游苗,進行4項試驗。實驗一比較在10 ~ 100 μE之5種光照度下,在玻片上增殖的3種矽藻與無矽藻的對照組,在原光照以培養皿養殖東東 (雌雄親貝為台東養殖的簡稱) 九孔苗,孵化後第2天 (2 days post hatch, dph) 對照組的活存 (86 ~ 95%) 高於矽藻組 (24 ~ 86%);10 dph變態完成期,對照組在10 ~ 75 μE有較高的活存 (8 ~ 23%),而2種矽藻組僅在高光照100 μE有活存 (4 ~ 5%)。實驗二在培養皿養殖南南九孔苗,添加迦瑪-安基丁酸 (GABA) 組第二天的活存 (39 ± 9%),較未添加組 (24 ± 5%) 佳;在第一天加GABA第二天投餵矽藻及每天更換海水和加抗生素組,其第三天後養殖期間活存的平均值,高於二種藥物都未添加組;6種矽藻中以扁圓卵形藻多孔變種 (Cocconeis placentula var. euglypta)最佳,20 dph 的活存為8 ± 10%。實驗三以相同的6種矽藻在培養皿增殖後,先洗除附著差的藻體,再移入東南九孔苗,每天以含抗生素海水更新,養殖到20 dph的活存 (52 ± 7%) 及22 dph的殼長(0.77 ± 0.14 mm),均以扁圓卵形藻為餌組顯著最高。實驗四增加至11種矽藻,同樣的方法在培養皿養殖和南九孔苗至13 dph時,以扁圓卵形藻、穀皮菱形藻 (Nitzschia palea) 及潛艇曲殼藻(Achnanthes submarina) 為餌組殼長 (0.53 ~ 0.57 mm) 顯著較高,但活存 (4.0 ~ 5.5%) 無差異。在3L壓克力槽打氣養殖到19 dph 時,6種矽藻中也以扁圓卵形藻為餌組殼長 (0.96 ± 0.14 mm) 顯著較高,而活存 (16 ± 6%) 也無差異。在250 L玻璃纖維水槽打氣養殖到20 dph時,3種矽藻中以潛艇曲殼藻為餌組每片浪板平均幼苗數 (511 ± 215隻/片) 及活存 (2.15%) 最高,殼長1.08 ± 0.25 mm。綜合上述試驗,以扁圓卵形藻及潛艇曲殼藻為餌的九孔苗有較佳的活存與成長。

摘要abstract(英)


To find the resolution of diet algae associated problems in abalone Haliotis diversicolor post-larvae rearing, four experiments were conducted using the post-larval abalone produced from different spawners: TT type (female and male all from Taitung), NN type (female and male all from Tainan), TN type (female from Taitung but male from Taitung) and HN type (female from Ho-ping Island, Keelung but male from Tainan). In experiment 1, the survival of TT post-larval abalone at the second day (2 days post hatch, 2 dph), cultured in Petri dish at 5 light intensities, was better in the blank (86 ~ 95%) than those fed with 3 diatoms on cover glass (24 ~ 86%). At 10 dph metamorphosis finished, higher survival (8 ~ 23%) was found in the blank at 10 ~ 75 μE, while 2 diatom groups survived (4 ~ 5%) only at 100 μE. In experiment 2, the survival of NN abalone post-larvae at the next day, cultured in Petri dish, was better (39 ± 9%) in the group addition with gamma-amino butyric acid (GABA) than the group without addition (24 ± 5%). The survival rates of abalone in the cultured periods after the 3rd day were better in the group, addition with GABA at 1st day then daily change of sea water with antibiotic and provided the diatom at 2nd day, than the group without both GABA and antibiotic addition. The best survival (8 ± 10%) at 20 dph was found in the group providing the Cocconeis placentula var. euglypta. In Experiment 3, the same 6 diatoms grew in Petri dish was washed to remove the floating algae, then the TN abalone post-larvae were put in and sea water with antibiotic changed daily, the significantly best survival (51.5 ± 7.3 %) at 20 dph and largest shell length (0.77 ± 0.14 mm) at 22 dph was found in the group fed with C. placentula var. euglypta. In Experiment 4, using the same rearing method, the better growth (0.53 ~ 0.57 mm) of the HN abalone post-larvae at 13 dph was in the groups fed with C. placentula var. euglypta, Nitzschia palea and Achnanthes submarina among 11 diatoms, but their survival (4.0~5.5%) was not significantly different. Reared in the 3-L tank with plastic plates and aeration, at 19 dph the abalone fed with C. placentula var. euglypta showed again the significantly best growth (0.96 ± 0.14 mm) but no different survival (16 ± 6%) among 6 diatoms. Reared in 250-L tanks with plates and aeration, at 20 dph the abalone on each plate (511 ± 215) and survival (2.15 %) was highest in the group fed with A. submarina, and the shell length was 1.08 ± 0.25 mm. In conclusion, two diatoms C. placentula var. euglypta and A. submarina showed the better survival and growth for post-larval abalone H. diversicolor.