農業部-水產試驗所全球資訊網農業部-水產試驗所全球資訊網

本研究旨在探討半葉馬尾藻萃取物以大鼠初代肝細胞之分離與培養為實驗模式，評估不同濃度之半葉馬尾藻萃取物對大鼠初代肝細胞的存活率、抗氧化及解毒代謝能力之影響。接著再將大鼠初代肝細胞與大鼠施以四氯化碳 (CCl₄) 誘導氧化損傷，評估半葉馬尾藻萃取物之護肝及抗氧化能力。結果發現，以5.0 mg/mL半葉馬尾藻萃取物培養大鼠初代肝細胞後，能夠顯著提升細胞存活率，並使胞內麩胱甘肽 (Glutathione, GSH) 含量與麩胱甘肽S轉移酶 (Glutathion-S-transferase, GST) 酵素活性上昇。而大鼠初代肝細胞受四氯化碳損傷時，半葉馬尾藻萃取物可以顯著增加大鼠初代肝細胞的存活率，且能增加正常大鼠初代肝細胞的GSH含量，並且提升抗氧化系統的麩胱甘肽過氧化酶 (Glutathione peroxidase, GPx)、麩胱甘肽還原酶 (Glutathione reductase, GRd)、過氧化氫酶 (Catalase, CAT)、超氧化物歧化酶 (Superoxide dismutase, SOD) 與解毒酵素GST的活性。除此之外，半葉馬尾藻萃取物也能降低因四氯化碳氧化損傷所引起的細胞壞死現象發生。在動物實驗方面，餵食半葉馬尾藻萃取物160 mg/kg body weight 時能夠降低麩胺酸-草醋酸轉胺酶 (Glutamic oxoaloacetic transaminase, GOT) 與麩胺酸-焦葡萄酸轉胺酶 (Glutamic pyruvic transaminase, GPT) 肝損傷指標，並進一步提昇GSH、GPx、GRd、SOD與GST酵素活性。最後由肝臟病理組織切片可得知，餵食半葉馬尾藻萃取物後可降低四氯化碳誘發之細胞空泡化及壞死細胞之生成。從上述結果得知，半葉馬尾藻萃取物可顯著降低肝臟損傷程度，並可顯著提升肝臟中因四氯化碳誘導下降之抗氧化酵素活性，因此推測半葉馬尾藻萃取物應是阻斷四氯化碳對細胞之氧化傷害而達到保護肝臟之效果。

The aim of this study was to investigate the effects of various concentrations of Sargassum hemiphyllum extract on cell viability, antioxidation system in primary rat hepatocytes and hepatic protection against carbon tetrachloride (CCl₄)-induced hepatic damage. The results showed that the primary rat hepatocytes had the maximum cell viability at 5.0 mg/ml and was significantly higher than negative control (p < 0.05). In addition, the treatment of S. hemiphyllum extract could increase the content of intracellular glutathione (GSH) and the activity of glutathione-S-transferase (GST) in primary rat hepatocytes. The S. hemiphyllum extract exhibited significant protective activity against CCl₄ induced hepatotoxicity in primary rat hepatocytes, and restored the level of cell viability, GSH content and the activities of antioxidant enzymes such as GST, glutathione reductase (GRd), glutathione peroxidase (GPx), superoxide dismutase (SOD) and catalase (CAT). Finally, the oral treatment with S. hemiphyllum extract for 8 consecutive weeks could cause significant reduction of both GOT and GPT levels after the co-administration of CCl₄ induced hepatic damage in rats. It was found that S. hemiphyllum extract could significantly decrease GOT and GPT. In antioxidant activity evaluation, administration of S. hemiphyllum extract also could significantly increase the GSH content and the activity of GPx, GRd, GST and SOD in liver. The histopathological evaluation of the liver also revealed that S. hemiphyllum extract reduced the incidence of liver lesions induced by CCl₄. Based on these result, we suggested that S. hemiphyllum extract exerted their hepatoprotective activities against CCl₄-induced injury by preserving the cellular antioxidative defense system.