農業部-水產試驗所全球資訊網農業部-水產試驗所全球資訊網

本研究利用石蓴醣溶液複合魷魚皮酵素水解物以產製高抗氧化能力的水產複方，其清除 DPPH 自由基能力、螯合亞鐵能力、還原力及 SOD-like 活性的EC50值分別為 0.54、4.52、1.61 及 5.98 mg/ml。另以 3T3-L1 前脂肪細胞 (preadipocytes) 評估其對脂質生成之影響，結果顯示水產複方 (2.5、5 及 10 mg/ml) 對 3T3-L1 前脂肪細胞不具細胞毒性，且於10 mg/ml 濃度下，具有抑制其分化為成熟脂肪細胞 (mature adipocytes) 之能力。在抑制脂肪堆積作用發現：2.5、5 及 10 mg/ml 的水產複方皆能抑制成熟脂肪細胞中脂質的蓄積、降低細胞內三酸甘油酯含量及甘油-3-磷酸去氫酶活性，且隨濃度愈高其抑制能力愈強。綜合上述結果顯示：水產複方具有良好的抑制脂肪細胞分化及降低脂質堆積的作用，應具有作為調節脂質生成之保健素材的發展潛力。

A marine formula composed of Ulva lactuca extraction and enzymatic hydrolysates was extracted from Dosidicus gigas skins. In vitro assay demonstrated that the marine formula possessed good antioxidative activities, EC50 (effective concentration of antioxidant substance removing 50% of free radicals) on DPPH free radical scavenging activity, chelating activity of Fe2+, reducing power and SOD-like activity were 0.54, 4.52, 1.61 and 5.98 mg/ml, respectively. The aim of this study was to evaluate the effect of the marine formula against lipid accumulation by using 3T3-L1 peradipocytes. At 2.5, 5 and 10 mg/ml, the marine formula demonstrated no cytotoxicity to peradipocytes; however, it could inhibit differentiation of mature adipocytes. In terms of lipid accumulation, the results showed that 2.5, 5 and 10 mg/ml of the marine formula could inhibit lipid accumulation, decreasing the triglyceride contents and glycerol-3-phosphate dehydrogenase activity in a dose-dependent manner.