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水產研究(1993年創刊)
台灣產8種梭子蟹類粒線體DNA中COI及16S rRNA之類緣關係研究
- 出版日期：2008-12-31
- 標題title(英)：Phylogenetic Analyses of Eight Species of Portuniids of Taiwan Based on DNA Sequences of Mitochondrial COI and 16S rRNA
- 作者：梁宏彥‧蕭聖代
- 作者auther(英)：Hong-Yen Liang and Sheng-Tai Hsiao
- 卷別：16
- 期別：2
- 頁碼：29-39
梭子蟹類是台灣重要的經濟水產資源之ㄧ，其資源管理往往因過度捕撈產生許多問題。然而物種基因序列的建立是進行種類的鑑定及親緣比對的重要工具，有了這些基礎方能進行完善的資源管理。因此，本研究利用粒線體DNA中16S rRNA和COI兩段基因之核苷酸序列資料，作為台灣產經濟梭子蟹類物種鑑定時分子遺傳標誌 (Molecular marker)，並了解其種內和種間的遺傳差異及探討其分類地位。本研究類緣分析結果發現，梭子蟹科依其不同屬可分為四群，第一群為蟳屬 (Charybdis) 的銹斑蟳 (C. feriatus) 和顆粒蟳 (C. granulate)，第二群為梭子蟹屬 (Portunus) 的遠海梭子蟹 (P. pelagicus) 和紅星梭子蟹 (P. sanguinolentus)，第三群為青蟹屬 (Scylla) 的欖綠青蟹 (S. olivacea)、鋸緣青蟹 (S. serrata) 和擬深穴青蟹 (S. paramamosain)，第四群則是圓趾蟹屬 (Ovalipes) 的細點圓趾蟹 (O. punctatus)。由親緣關係樹狀圖可以發現蟳屬和梭子蟹屬關係較為接近與青蟹屬關係較遠。
摘要abstract(英) Portuniids is the most abundant decapod and also an important resource in crustacean fisheries in Taiwan. However, overfishing has resulted in a decline in the resource. Species identification for fishery resources management, trade and conservation of aquatic species are very important because identify taxa correctly and unambiguously are fundamental. The study was to develop a reliable database that can be used to distinguish different swimming crab species. Mitochondrial DNA 16S rRNA fragments and full sequence of COI gene of 8 crabs from Taiwan were amplified via PCR, and then were purified and sequenced. The phylogenetic tree constructed by using Neighbor-joining method showed that the relationship between genus Portunus and genus Charybdis is more closer than genus Scylla.
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- 水產研究16(2)台灣產8種梭子蟹類粒線體DNA中COI及16S rRNA.pdf
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