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水產研究(1993年創刊)
龍鬚菜之洋菜膠萃取與藻渣糖化處理
- 出版日期：2009-06-30
- 標題title(英)：Algal Polysaccharides Extraction and Algal Residues Saccharification of Gracilaria sp.
- 作者：范繼中‧李雅琳‧盧雅雯‧吳純衡
- 作者auther(英)：Chi-Chung Fan, Ya-Lin Lee, Ya-Wen Lu and Chun-Heng
- 卷別：17
- 期別：1
- 頁碼：81-91
龍鬚菜 (Gracilaria sp.) 是台灣養殖產量最高的藻類，其碳水化合物含量76.27% (乾基，粗纖維佔其中的8.41%)，經萃取海藻多醣後的藻渣，可以進行糖化反應轉化成糖液，作為生質酒精發酵原料之用。本研究以反應曲面法分析，獲得海藻多醣最適萃取條件：先以0.02 N硫酸溶液浸泡乾燥龍鬚菜0.3 hr後，再以100°C沸水萃取海藻多醣0.3 hr；以龍鬚菜海藻多醣含量為基準，計算其萃取率可達64.8%。以苯酚–硫酸法分析萃出液的總醣量比較，此萃取法可高於直接沸水萃取法的3倍以上。剩餘藻渣仍含有高量碳水化合物 (乾基，60.93%，其中粗纖維佔23.91%)，藻渣分別以酸水解、纖維素酶水解、以及先纖維素酶後酸水解處理，試驗其糖化效果，結果顯示經1% 纖維素酶處理的藻渣水解液，再以0.9 N硫酸處理，於121 ℃ 於殺菌釜中加熱1 hr的糖化產率最高；glucose以藻渣粗纖維量、galactose以藻渣海藻多醣量為計算基準，所得水解產率分別為95.0% 與43.5%。
摘要abstract(英) Gracilaria sp. is the most productive alga cultured in Taiwan. It contains 76.27% carbohydrate (dry-base weight, 8.41% crude fiber among them). After algal polysaccharides extraction, the remaining residues could be used as the source of bioethanol through saccharification treatment. The response surface methodology was applied to obtain an optimal agar extraction method that dried Gracilaria was immersed in 0.02-N sulfuric acid solution for 0.3 hr, and then extracted with boiling water (100oC) for 0.3 hr. Base on the algal polysaccharides content in the collected Gracilaria sp., the algal polysaccharides extraction rate was calculated to be 64.8%; this method has threefold higher extraction rate than the direct boiling water extraction method. The residues of algae residues still contained a high amount of carbohydrate (dry-base weight, 60.93%, which contained 23.91% cellulose). The algal residues were treated with acid, cellulase or cellulase-then-acid to assess their saccharification efficiencies. The results showed that the highest saccharification efficiency was observed when the algal residues were treated with 1.0% cellulase and then treated with 0.9 N sulfuric acid, which was heated in an autoclave at 121°C for 1 hr. Based on the algal residues crude fiber and algal polysaccharides,, the hydrolysis yields of glucose and galactose were calculated to be 95.0% and 43.5%, respectively.
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