農業部水產試驗所全球資訊網

一.海水觀賞生物之繁、養殖試驗（一）庫達海馬的每次生產卵數有限，一般數量在數百至一千多，若希望進行量產養殖，勢必蓄養為數不少的種魚，方能提供足夠的種苗生產。除了每次生產種苗數量少外，雌雄配對及交配是否成功，亦是一個限制種苗生產量的重要因素。因此，有必要在人工養殖環境下建立一個良好的庫達海馬雌雄配對機制。計畫利用各種不同的物理機制調控及配對比，提升庫達海馬配對的比例及交配成功率，同時配合良好的種苗養殖技術，應能建全庫達海馬的養殖技術。二.台灣重要河川魚介類遺傳資源分析與評估AFLP分析技術於1995年研發後，已成為近年來研究趨勢；本研究擬利用HindⅢ及EcoRⅠ限制酶在核酸上不規則分佈，並以特定接引子(Adapter)處理後，利用緊臨限制酶切點的2個任意核苷酸作為篩選，以225組逢機組合進行PCR反應，擴增DNA片段於電泳膠上呈現一特定的基因圖譜，此技術平台可作為種內與種間之分析研究，亦可作為種源庫資訊與參考。三.礁岩性觀賞魚類繁養殖開發1.完成白條海葵魚和克氏海葵魚之優良種苗繁殖技術。2.完成白條海葵魚和克氏海葵魚之生殖行為及育苗研究報告。3.確立海水觀賞魚類繁殖養殖技術，並提供相關技術給業者作為其他海水魚繁殖之參考。4.培育白條海葵魚種魚20尾，生產白條海葵魚仔魚2500尾。5.培育克氏海葵魚種魚20尾，生產克氏海葵魚仔魚2500尾。四.澎湖海域重要水產生物種原之培育研究本計畫之目的為執行澎湖章魚、象牙鳳螺及豹鱠之種原採集及蓄養，探討其於人為環境下產卵機制。採卵成功後繼續研究孵化及幼生培育技術，研究受精卵發育及孵化機制、幼生餌料及培育方法，建立種苗生產技術。同時也進行澎湖章魚棲地環境調查，提供人為環境繁養殖參考及地方政府漁業管理單位訂定澎湖章魚漁業資源管理政策。豹鱠(Plectropomus leopardus)俗稱七星斑，由於肉質細嫩鮮美，需求量相當大，天然海域捕獲量早已供不應求，是極具發展潛力之高經濟魚種。但因天然種苗資源量少，無法充分取得魚苗供應養殖，為解決此問題，本計畫將配合國家水產生物種原庫之興建，培育優質種魚，生產良質受精卵，突破魚苗培育瓶頸，生產種苗提供養殖事業發展，並鞏固我國石斑魚苗生產之國際領先地位。

研究報告摘要(英) 一.海水觀賞生物之繁、養殖試驗The number of the eggs spawned by seahorses each time is limited. The average numberis from many hundred to more than one thousand. If we want to make the massproduction of seahorses, we have to culture many seeds in order to supply enough fries.Besides the production of the seeds is numbered, whether mating is successful or not isanother key factor of limiting the production of the fries. Therefore, it is necessary tobuild a good matching model under the circumstances of aquaculture. We will usedifferent environmental factors and mating rate to promote the proportion ofseahorses（Hippocampus kuda） matching and the proportion of successful matching.We can adopt the good skills of the seed production at the same time, and thus we canbuild a good production technique of seahorses.二.台灣重要河川魚介類遺傳資源分析與評估AFLP technique, developed by Vos et al. (1995), is based on the selective PCR amplifiedof restriction fragments from a total of genomic DNA. To research and identifynumbers of AFLP markers, 225 primers combinations, base on the restriction enzymeEcoRI and HindⅢ with two base pair extensions, were tested to generate AFLP pattern.The markers are suitable for use in gene pool and trait loci by using the inter- and intraspecies.Analysis of genetic variations among strains of fish indicated that AFLP shouldalso be a valuable tool for genetic resource and population analysis in fish.三.礁岩性觀賞魚類繁養殖開發1.Excellent fry propagation techniques for the clown fish Ampfiprion frenatus andAmphiprion clarkii were established.2.Reproductive behavior and propagation research report for Ampfiprion frenatus andAmphiprion clarkii were finished.3.Establishment of aquaculture and propagation techniques for marine aquarium fish.We provided related techniques forthe industry as a reference for propagation for other marine fishes.4.There were 20 brood fishes for the clown fish Ampfiprion frenatus and 2500 fries wereproduced.5.There were 20 brood fishes for the clown fish Ampfiprion clarkii and 2500 fries wereproduced.四.澎湖海域重要水產生物種原之培育研究The objectives of this study are establishment of genetic stock and cultural techniques ofIvory shell, Babylonia areolata, Octopus spp.andcoral trout, Plectropomus leopardus.The stock will be collected and preserved for utilization of researchers. The tank system,diet, growth, mature size, spawning season, embryonic development, hatching, andlarvae rearing will be studies. The knowledge will be very valuable for the mass seedproduction. It is also needed to study monitoring and stock assessment on Octopus spp.fishery resource in the water of Peng-Hu. These investigated data will provide the fisheryadministration to establish the management policy for Octopus spp. fishery.The coral trout, Plectropomus leopardus, is an important commercial species and verymuch relished sea food in Taiwan. As the supply of wild fingerlings is limited anduncertain. The growth of the grouper farming industry in the region has been hamperedby the shortage of fingerlings. There is therefore a need to establish breeding techniquefor mass production of coral trout fingerlings. We have to study the development ofembryos, larval rearing, established the broodstock of parent fish, and keep good qualitygenetic strain in a role of aquaculture. For improve the techniques of coral trout cultureand produce mass seed.