農業部水產試驗所全球資訊網

近年來從海藻中萃取多種的生理活性物質，當作天然抗菌劑及食品防護劑，已引起國際間高度興趣。。本研究主要利用20種海藻，以甲醇、丙酮及水萃取其機能性成分,再分別測定其總酚含量及抗菌效果。抗菌效果之測試首先使用挖洞法（well diffusion assay）篩選20種海藻不同溶劑萃取液抑制微生物之效果，選出效果較佳者，繼續測試抑菌後之生長曲線，最小抑制菌濃度等，分析其抑菌效果，藉以評估海藻萃取物質對皮膚及食品致病菌之有效抑菌範圍。結果顯示20種海藻利用不同方法萃取，各具有不同之抑菌效果，尤其以丙酮及甲醇溶劑萃取，效果優於酵素及水萃取。海藻中主要抗菌物質為酚類化合物，因此評估其總酚及抗菌活性之相關性，進一步探討海藻抗菌之活性物質，將來可應用於製作海藻抗菌護膚產品及添加於食品中，取代合成抗菌劑之添加，避免造成人體之傷害。

研究報告摘要(英) Recently, numbers of researches associated with seaweed extracts to bioactivity effects, including antioxidation, antimutation, enhancing immunization, antibacterial, and antiviral abilities, have been come to notice internationally. As a result, development of extract from plants, especially in seaweeds, as nature antibacterial agents or food protection mediators could be the major topic in food sanitation researches. This study is assessed functional components, total phenolic content and antibacterial effect among 20 spices of seaweeds with different extracting solvents, including methanol, acetone, and water. Antibacterial effect is pre-screened with well diffusion assay among all of seaweed extracts, followed by evaluation beneficial tests on skin or food protection with bacterial generation curve and least inhibitory concentration methods for assessment outcomes of selected seaweed extracts. The result shows that 20 spices of seaweeds with different extracting had different antibacterial effects, actone and methanol extracts were better than enzyme and water extracts. The major antibacterial component in seaweed was phenolic. Hence, it is necessary to analysis the relationship between total phenolic content and antibacterial results. With outcomes of this study, it is expected that develops favorable seaweed extracts as a natural antibacterial skin-proofs or food additives substituting artificial protective mediators which they might result in damage to human.