農業部水產試驗所全球資訊網

透過光學來定量海中葉綠素濃度已經催生出許多先進科技的商業與研究用途。這些儀器運用了螢光、光吸收和散射等原理，能夠以高時間分辨率和快速採樣率來定量各種物質。然而，實驗所需的葉綠素甲 (Chlorophyll-a, Chl-a) 標準品價格昂貴且需低溫保存，增加了成本與不便性。本研究旨在替葉綠素測定尋找更穩定且經濟的標準品，為此比較三種螢光化學物質-羅丹明 WT (rhodamine WT, RWT)、螢光素鈉鹽 (fluorescein sodium salt, FSS) 和Chl-a在不同濃度下的螢光強度表現。本研究使用微孔板分析儀測量這些化合物的螢光反應，並分析其穩定性與靈敏度。結果顯示，FSS在所有濃度下表現出最佳的穩定性與區分能力，而RWT在高濃度下出現輕微重疊，但仍具應用潛力。相比之下，Chl-a在高低濃度下的螢光反應變異性較大。FSS的穩定螢光表現顯示出它作為Chl-a標準品替代品的潛力。這些發現為未來螢光檢測標準品的選擇提供了指引，特別是在環境科學與生物監測領域的應用中。

摘要abstract(英) Optical methods for quantifying chlorophyll-a (Chl-a) concentrations in marine environments are crucial for commercial and research applications. Instruments leveraging fluorescence, light absorption, and scattering offer high temporal resolution and rapid sampling for dissolved and particulate matter. However, calibrating with Chl-a standards is costly and requires cold storage, posing significant experimental challenges. This study compares the fluorescence intensity performance of three compounds ‒ Rhodamine WT (RWT), Fluorescein Sodium Salt (FSS), and Chl-a ‒ at various concentrations. Using a microplate reader under controlled conditions, we measured their fluorescence responses, analyzing stability and sensitivity. FSS demonstrated superior stability and distinguishability across all concentrations. While RWT showed slight overlap at high concentrations, it remains a promising candidate. In contrast, Chl-a exhibited greater variability in fluorescence response at both high and low concentrations. The stable fluorescence performance of FSS suggests its strong potential as a substitute for Chl-a standards. These findings offer valuable guidance for selecting fluorescence standards, particularly in environmental science and biological monitoring.