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研究計畫及成果
水產種苗研究團隊－生藥應用於魚蝦病防治研究
- 計畫編號：99農科-10.3.1-水-A4(1)
- 年度：2010
- 領域：漁業科技研發領域
- 主持人：郭錦朱
大蒜在很早以前已被證實具抗菌、除黴及抗病毒之功能，但拮抗海鱺主要致病原發光桿菌的相關研究並不多。本研究旨在探討大蒜在生體外及生體內對海鱺發光桿菌的拮抗能力。大蒜在生體外之拮抗活性是以最小抑菌濃度及抑菌環來評估；結果發現，大蒜水萃液對發光桿菌具拮抗活性，其最小抑菌濃度為10 mg/ml。在生體內的抗菌活性，則是以大蒜飼料投餵海鱺，再以發光桿菌攻擊，結果發現，大蒜能有效減少海鱺罹發光桿菌症之死亡率 (p<0.05)。
研究報告摘要(英) Garlic, Allium sativum, has long been known to have antibacterial, antifungal and antiviral properties but there are few data on its effects against Photobacterium damselae subsp. piscicida which is one of the major pathogenic bacteria of cobia, Rachycentron canadum. The in vitro activity of garlic extract was assessed by determination of minimum inhibitory concentration (MIC) and zone diameter. The aqueous garlic extract were found to be able to inhibit growth of bacteria in a dose-dependent manner with maximum of 100% at defined concentration. The MIC for P. damselae subsp. piscicida was 10 mg/ml. The in vivo efficacy of garlic extract after P. damselae subsp. piscicida experimental infection of cobia was evaluated. The garlic diet fed group got improved survival (p<0.05).