研究計畫及成果
鯖魚頭開發貓類機能性飲品之研究(I)
- 計畫編號：108農科-14.1.4-水-A1
- 年度：2019
- 領域：農業生物經濟
- 主持人：高翊峰
- 研究人員：高淑雲、易琮凱、杜明杰
隨著國人飼養家貓作為伴侶動物的風氣日漸增加，家貓的保健營養亦逐漸受到重視 。慢性腎臟病、關節炎及腹膜炎等是家貓常見的發炎相關疾病，然而研究家貓免疫 調節相關保健的文獻確相對匱乏。本篇研究建立初代培養之家貓周邊血液單核球細 胞(feline-PBMC)及類巨噬細胞(feline-MÆ)試驗，評估鯖魚頭磷脂質製成之微脂體 生物製劑(MH-Lip.) 對家貓免疫調節之可行性。鯖魚頭磷脂質組成包含：3.6 %磷脂 醯肌醇(phosphatidylinositol, PI)、44.9 %磷脂醯膽鹼(phosphatidylserine, PC)、30.5 %磷脂醯乙醇胺(phosphatidylethanolamine, PE)、9.7 %磷脂醯絲胺酸 (phosphatidylserine, PS)、0.6 %溶血磷脂膽鹼(lyso-phosphatidylcholine, lyso-PC)及10.8 %其他脂質，加工製成微脂體後粒徑約180 nm，其外層膜電位53mV；添加3 mg/mL以下的MH-Lip.共培養，對feline-PBMC或feline-MÆ細胞無顯著 毒性;在脂多醣誘導feline-PBMC細胞發炎實驗中，添加0.33~3 mg/mL MH-Lip.顯著 調降促發炎細胞激素fIL-1b、fIL-12及fTNF-a分泌(P<0.05)；在脂多醣誘導felineMÆ發炎實驗中，添加1~3 mg/mL MH-Lip.顯著調降fIL-1b、fIL-6、fIL-12及fTNFa促發炎細胞激素分泌及mRNA的表現(P<0.05)；此外在LPS誘導老鼠巨噬細胞 (RAW264.7)的氧化壓力試驗中，添加鯖魚頭生物製劑亦顯著減少氧自由基生成 (P<0.05)。綜合上述結果顯示：鯖魚頭生物製劑應具調節家貓血液單核球細胞免疫 機能，並能有效緩解發炎所導致的氧化壓力，未來應有助於發展家貓保健飲品。
研究報告摘要(英)
In this study, the cell based assays from primary feline peripheral blood monocyte cells (feline-PBMC) and macrophage liked cell (feline-MÆ) were established firstly, and the assays were used to evaluate whether the phospholipid liposome from mackerel hear prepared (MH-lip.) regulated the inflammatory feline immune cells. The components of mackerel phospholipids were including 3.6 % of phosphatidylinositol (PI), 44.9 % of phosphatidylcholine (PC), 30.5 % of phosphatidylethanolamine (PE), 9.7 % of phosphatidylserine (PS), 0.6 % of Lyso-phosphatidylcholine (Lyso-PC) and 10.8% of others. The particle size and zeta potential of MH-lip. were measured at 180 nm and -53 mV, respectively. Treatment below 3 mg/mL of MH-lip showed no significant cytotoxicities on feline-PBMC and feline-MÆ cells. Co-treated with 0.33~3 mg/mL of MH-lip. had decreased proinflammatory cytokines secretion (P<0.05) including fIL-1b, fIL-12 and TNF-a on LPS stimulated feline-PBMC cells. Co-treated with 1~3 mg/mL of MH-lip. not only decreased pro-inflammatory cytokine secretions (P<0.05) but also decreased mRNA expressions (P<0.05), including fIL-1b, fIL-6, fIL-12 and TNF-a on LPS stimulated feline-PBMC cells. Furthermore, treatment of MH-lip was showed remarkable suppressed reactive oxide - 1 - 1082389 1. 2. 3. species production on LPS induced RAW264.7 cells. Conclusion that MH-lip regulated the immune functions of feline PBMC and feline-MÆ cells, also provided anti oxidative stress, those data will assist MH-lip. application on development of feline functional beverage products.