Genetic improvement plays an important role in sustainable development for Haliotis diversicolor aquaculture industry. This aim of the project will focus on investigating the genetic variation of the main cultured H. diversicolor in Taiwan and select molecular markers with cold-tolerant to establish superior economical traits of broodstocks. The RAPD primers were used to analyze the genetic characteristics of the H. diversicolor sampled over recent years. Some abalone groups had several missing loci, and even the entire genetic locus disappeared, which indicates that the artificial genetic breeding has been caused by the process of artificial breeding. In addition, through the low-temperature screening module established in this study, the short-term (40 days) survival rate can reach 90%; and in the reproductive season, it is stored at a lower temperature, even if the gonads are mature, it is not easy to have a collective ejaculation. Moreover, the feeding status of the test group was not different from that of the control group, and the long-term (>300 days) low temperature storage was observed, and the gonads of the temperature control group were not synchronized.